Figure 1-6938.

Expression of CXCR4 on freshly isolated CD16⁺CD56⁺ human peripheral NK cells. This figure shows the results of purified NK cells using a magnetic separator as described in Materials and Methods. Panel I shows the purified CD16⁺CD56⁺ NK cells; more than 85% of NK cells were CD16⁺CD56⁺. Panel II: The CD16⁺CD56⁺ NK cells were further purified and stained with anti-CD29 (β₁ integrin) (A), anti-CD49b (α₂ integrin) (B), or anti-CXCR4 antibodies (C) as described in Materials and Methods. The dotted lines represent the patterns of staining with the secondary antibody only. Results shown are for one donor (the same donor shown in other assay) but are representative of 12 donors tested.