Figure 1-6915.

In vitro interaction between NUB1 and synphilin-1. GST and GST-fused HHR23B and NUB1 were expressed in bacteria and purified using glutathione-Sepharose beads. RH-tagged synphilin-1 (sph1) expressed in bacteria was then precipitated with these beads, which were coated with GST alone (lane 2), GST-HHR23B (lane 3), or GST-NUB1 (lane 4). The precipitates were analyzed by Western blotting using anti-RH antibody to detect RH-synphilin-1 (top) and anti-GST antibody to detect immobilized GST, GST-HHR23B, or GST-NUB1 (bottom). In lane 1, the input of RH-synphilin-1 diluted to 1:40 was loaded as a control. Molecular size markers are shown in kilodaltons.