Figure 2-6922.

CYR61 is increased in the dermis of sun-protected chronologically aged human skin in vivo. A: Skin samples were obtained from chronologically aged (80+ years) and young (20 to 30 years) individuals. Dermis was captured by LCM, and CYR61 and 36B4 (internal normalization control) mRNA levels were quantified by real-time RT-PCR. Data are means ± SEM, n = 6, *P < 0.05. B: Dermis was separated from epidermis by dissection. Dermal CYR61 and β-actin (internal normalization control) protein levels were determined by Western analysis. Inset shows representative Western blot. Data are means ± SEM, n = 8, *P < 0.05.