Schematic representation of a Zepp element, ZA-1 (A), and the terminal Zepp cluster in miniV4 (B). (A) The full-length Zepp element ZA-1 consists of a 779 bp 5′ untranslated region (5′UTR), ORF1 (1839 bp), ORF2 (3927 bp) and a 2399 bp 3′ untranslated region (3′UTR) with a poly(A) tail of varying size (16). The 800 bp HindIII fragment was used as a probe for Southern blotting analysis. (B) Zepp cluster on the miniV4 terminal region. The 0.9 (L), 0.6 (M) and 0.3 kb (S) fragments detected in Figure 2A contain repeated 3′-regions of Zepp elements in a tandem array with their poly(A) tails towards the centromere. Numbers indicate positions corresponding to the ZA-1 sequence.