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. 2006 Sep;169(3):815–822. doi: 10.2353/ajpath.2006.060037

Figure 5.

Figure 5

ET-1 potently activates skin MCs in vitro via ETA. A: Unpurified (∼3% of all cells) or purified (>90%) skin MCs were obtained from C57BL/6 mice and degranulation was assessed by measuring serotonin release after stimulation with ET-1 or IgE + Ag. Calcium ionophore (A23187; 10−5 M) was used as positive control. Data are corrected for spontaneous release, which was <10% in all experiments. B: Inhibition of ET-1-induced (10−6 M) degranulation of unpurified skin MCs (∼3% of all cells) by selective antagonists for ETA (BQ-123) or ETB (BQ-788) or vehicle as assessed by measuring serotonin release. Data are corrected for spontaneous release (<14% in all experiments). Data are pooled from three (B) or two (A) independent experiments. All data shown as means ± SEM. **P = 0.01, ***P = <0.005. n.d., not detectable.