Figure 1.

Bleomycin-induced lung fibrosis was augmented in E-selectin^−/− mice and E-selectin^−/− mice treated with anti-P-selectin. A: Representative histological sections of lungs from wild-type, P-selectin^−/−, E-selectin^−/−, and E-selectin^−/− mice treated with anti-P-selectin mAb 16 days after intratracheal bleomycin administration. Wild-type mice that received intratracheal saline injection served as controls (control). The lung sections were stained with H&E to evaluate alveolitis and with Azan-Mallory to identify collagen deposition. B: Lung fibrosis was evaluated according to the criteria described in Materials and Methods. The results are indicated as a mean ± SEM of eight mice in each group. Original magnifications, ×100.