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p3′luc is a derivative of pBestluc, and is a high copy vector that expresses firefly luciferase (clear box) from the tac promoter (shaded box) in bacteria. This vector has unique PacI and ClaI restriction sites situated in the 3′ end of the luciferase coding region. A double-stranded oligonucleotide was inserted at the XhoI site to establish a direct repeat sequence (hatched box). The arrows indicate the position of DNA primers used for PCR analysis.
