Table 2. Double-stranded oligonucleotides containing two uracil lesions.
Name | Position of two Us relative to each other | Sequence | |
---|---|---|---|
5′ or 3′ | Base pairs apart | ||
U – 34 | 5′ | 33 | 5′ TAAATACAAAGGATATCAGGTGGCCCCCGCTGAATUGGAAT 3′ |
3′ TAAUTTATGTTTCCTATAGTCCACCGGGGGCGACTTAACCTTAGC 5′ | |||
U – 21 | 5′ | 20 | 5′ TAAATACAAAGGATATCAGGTGGCCCCCGCTGAATUGGAAT 3′ |
3′ TAATTTATGTTTCCTAUAGTCCACCGGGGGCGACTTAACCTTAGC 5′ | |||
U – 19 | 5′ | 18 | 5′ TAAATACAAAGGATATCAGGUGGCCCCCGCTGAATTGGAAT 3′ |
3′ TAAUTTATGTTTCCTATAGTCCACCGGGGGCGACTTAACCTTAGC 5′ | |||
U – 14 | 5′ | 13 | 5′ TAAATACAAAGGATAUCAGGTGGCCCCCGCTGAATTGGAAT 3′ |
3′ TAAUTTATGTTTCCTATAGTCCACCGGGGGCGACTTAACCTTAGC 5′ | |||
U – 8 | 5′ | 7 | 5′ TAAATACAAAGGATATCAGGUGGCCCCCGCTGAATTGGAAT 3′ |
3′ TAATTTATGTTTCCUATAGTCCACCGGGGGCGACTTAACCTTAGC 5′ | |||
U – 6 | 5′ | 5 | 5′ TAAATACAAAGGATATCAGGUGGCCCCCGCTGAATTGGAAT 3′ |
3′ TAATTTATGTTTCCTAUAGTCCACCGGGGGCGACTTAACCTTAGC 5′ | |||
U – 1 | 5′ | 0 | 5′ TAAATACAAAGGATAUCAGGTGGCCCCCGCTGAATTGGAAT 3′ |
3′ TAATTTATGTTTCCTAUAGTCCACCGGGGGCGACTTAACCTTAGC 5′ | |||
U + 1 | 3′ | 0 | 5′ TAAATACAAAGGAUATCAGGTGGCCCCCGCTGAATTGGAAT 3′ |
3′ TAATTTATGTTTCCTAUAGTCCACCGGGGGCGACTTAACCTTAGC 5′ | |||
U + 8 | 3′ | 7 | 5′ TAAAUACAAAGGATATCAGGTGGCCCCCGCTGAATTGGAAT 3′ |
3′ TAATTTATGTTTCCUATAGTCCACCGGGGGCGACTTAACCTTAGC 5′ |
When the two uracils are positioned 5′ to each other, the name is designated with a minus and when they are 3′ to each other, the name is designated with a plus. The number in the name designates the nucleotide position of the second uracil relative to the first.