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. 2006 Sep;169(3):831–845. doi: 10.2353/ajpath.2006.051237

Figure 5.

Figure 5

Validation of siRNA targeting bmi1. A: bmi1 mRNA expression was significantly lower in BECs transfected with siRNA targeting bmi1 on days 2, 3, and 4. bmi1 mRNA was quantified with real-time PCR. The expression was normalized as a ratio using GAPDH as a housekeeping gene. Data are expressed as the mean ± SD. *P < 0.01, **P < 0.05 compared to the control, n = 3 for each group. B: Immunoblot analysis for bmi1 protein expression in BECs transfected with siRNA targeting bmi1 and a control siRNA. Day 3 after transfection. Three independent experiments showed similar results. C: bmi1 expression labeled by red fluorescence was suppressed in the cells transfected with bmi1 siRNA showing green fluorescence in the cytoplasm (arrows) (left). In contrast, bmi1 expression was not decreased in the cells transfected with control siRNA (right). Day 3 after transfection.