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. 2006 Sep;169(3):831–845. doi: 10.2353/ajpath.2006.051237

Figure 7.

Figure 7

The knockdown of bmi1 inhibits cell proliferation and induces cellular senescence. A: Cell proliferation was assessed by 5-bromo-2′-deoxyuridine (BrdU) assay. The labeling index of BrdU was counted for at least 1000 cells in each group. The labeling index of BrdU was significantly low in BECs transfected with bmi1 siRNA, when compared with BECs transfected with control siRNA. Data are expressed as the mean ± SD. *P < 0.01. Day 4 after transfection. B: Most BECs transfected with bmi1 siRNA showing green fluorescence in cytoplasm were negative for BrdU-labeling (red fluorescence). C: Cellular senescence was assessed by the senescence-associated β-galactosidase activity (SA-β-gal). Percentage of cells for positive for SA-β-Gal activity was significantly higher in BECs transfected with bmi1 siRNA, when compared with BECs transfected with control siRNA. Data are expressed as the mean ± SD. *P < 0.01. Day 6 after transfection. D: Number of colonies was significantly lower in BECs transfected with bmi1 siRNA, when compared with BECs transfected with control siRNA. Data are expressed as the mean ± SD. *P < 0.01. Day 6 after transfection.