The effect of oxidative stress, UV irradiation, and serum deprivation on EZH2 expression in BECs. A: EZH2 mRNA expression was significantly lower in BECs on day 4 after H2O2 (112.5 μmol/L, 2 hours) treatment. B: EZH2 mRNA expression was significantly lower in BECs on days 1 and 2 after serum deprivation and on day 2 after UV irradiation (5 J/m2). A and B: EZH2 mRNA was quantified with real-time PCR and the expression was normalized as a ratio using GAPDH as a housekeeping gene. Data are expressed as the mean ± SD. *P < 0.01 compared to the control, n = 3 for each group. Serum dep, serum deprivation.