Table 2. Plasmids.

The details for the construction of plasmids are given in Materials and Methods. The main characteristics (origins of replication, antibiotic resistance genes) are indicated. The table has been subdivided in three sections. Transposable modules: pIROK and pIRKP carry the transposable modules derived from IS1397 and ISKpn1, respectively. IS1397 and ISKpn1 transposase plasmids: counter selectable markers and the type (OrfA or OrfAB) and the origin (IS1397 or ISKpn1) of the transposase are indicated. All plasmids express lacI, which allows the repression of the transposases in the absence of IPTG. Target plasmids: they all derive from pGEM®-T (Promega). The origin of the inserts (either a PCR or a double stranded DNA fragment) is indicated as well as their structure. The boxes indicate the PUs and the triangle indicates their orientation. White boxes, K.pneumoniae (K.p.) Z¹ PUs; gray boxes, E.coli (E.c.) Z² PUs; black boxes, E.coli Y PUs.