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. 2006 Oct;169(4):1303–1311. doi: 10.2353/ajpath.2006.060102

FIGURE 1.

FIGURE 1

Twist haploinsufficiency reduces Cbl expression in cranial osteoblasts. A: Total RNA from calvaria osteoblasts from a coronal suture in an SCS patient with Y103X Twist mutation (M-Tw) and wild-type (WT) human calvaria osteoblasts was subjected to RT-PCR analysis to detect Cbl levels, and GAPDH was used as internal control. B: Western blot analysis showing decreased Cbl protein levels in M-Tw cells compared with WT cells. C and D: M-Tw cells were transfected with a Twist expression vector or control vector (pcDNA3), and Cbl mRNA levels were determined. Transient transfection with Twist increased Cbl mRNA levels compared with cells transfected with the empty vector. D: Western blot analysis confirmed the increased Cbl protein levels in Twist-transfected M-Tw cells compared with cells transfected with the empty vector.