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Immunohistochemical analysis of PI3K in human coronal sutures in patients with SCS. Coronal sutures in SCS patients with Twist mutations (Y103X, Q109X, R118C) inducing Twist haploinsufficiency showed increased total PI3K (p85) immunoreactivity in osteoblasts (arrows) compared with normal sutures from age-matched controls. Original magnifications, ×125.
