FIGURE 6.

Forced expression of Cbl restores PI3K/Akt signaling in Twist mutant osteoblasts. M-Tw calvaria osteoblasts were transfected with Cbl expression vector or control vector (pBK) for 48 hours and cell lysates were subjected to Western blot to detect total and phosphorylated (ph)-PI3K (A) or ph-Akt (B), and the data were compared with the levels in wild-type (WT) osteoblasts. C: The levels of PI3K and ph-PI3K were measured by scanning densitometry and corrected for β-actin. The data are the mean ± SEM of five (PI3K) or three (ph-PI3K) different experiments.