Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
TGF-β1 mediated activation of signaling pathways. Confluent monolayers of HK2 cells were stimulated by the addition of TGF-β1 (1 ng/ml) for up to 6 hours. At the time points indicated, total cell extracts were generated, and immunoblot analysis of lysate samples for phosphorylated-Smad/total Smad (A), phosphorylated ERK/total-ERK (B), and phosphorylated p38/total p38 (C) was performed.
