TABLE 4.
Validation of Selected Differential Genes by Real-Time RT-PCR
| Transcript | Cell line | Flat | Three-dimensional | t-test |
|---|---|---|---|---|
| GDF15 | MUM2b | 24.2 (SD ± 0.23, n = 6) | 20.1 (SD ± 0.62, n = 5) | P < 0.0001* |
| M619 | 25.98 (SD ± 1.018, n = 4) | 20.2 (n = 1) | ||
| OCM-1 | 19.4 (SD ± 0.23, n = 4) | 20.7 (SD ± 1.089, n = 4) | P = 0.06 | |
| ATF3 | MUM2b | 23.42 (SD ± 0.53, n = 6) | 21.72 (SD ± 0.32, n = 5) | P = 0.0014* |
| M619 | 25.0 (SD ± 0.21, n = 4) | 20.8 (n = 1) | ||
| OCM-1 | 21.15 (SD ± 0.28, n = 4) | 23.15 (SD ± 1.1, n = 4) | P = 0.0184* | |
| CDKN1A | MUM2b | 21.52 (SD ± 0.19, n = 6) | 20.72 (SD ± 0.57, n = 5) | P < 0.01* |
| M619 | 26.53 (SD ± 0.12, n = 4) | 23.7 (n = 1) | ||
| OCM-1 | 23.88 (SD ± 0.20, n = 4) | 25.83 (SD ± 1.49, n = 4) | P = 0.04* | |
| CD44 | MUM2b | 17.4 (SD ± 0.49, n = 6) | 18.28 (SD ± 0.31, n = 5) | P < 0.0079* |
| M619 | 18.33 (SD ± 0.22, n = 4) | 19.40 (n = 1) | ||
| OCM-1 | 18.53 (SD ± 0.22, n = 4) | 19.38 (SD ± 0.47, n = 4) | P = 0.0172* |
Higher Ctvalues indicate lower expression levels and vice versa.
*
Student’s t-test, P value significant (P < 0.05).