Table 5.

Priority ranking of candidate genes

Gene symbol	Build 34 (bp)	Haplotype block	cSNPs known	Allele	Function	Human ortholog location	Human phenotype
High priority
Csk	57,740,571–57,759,105	Discordant	rs33644792	P312H	+	Chr15:72,861,767–72,882,557	No known phenotype
Adpgk	59,318,498–59,343,126	Discordant	rs13480222	A318T	+	Chr15:70,830,762–70,863,114	No known phenotype
Thsd4	60,087,012–60,259,136	Discordant	rs6224703a	V410M	+	Chr15:69,807,941–69,858,019	No known phenotype
Medium priority
Il18	50,637,695–50,654,164	Discordant	None known	NA	+	Chr11:111,519,186–111,540,050	Obesity
Cyp19a1	54,269,958–54,297,852	Discordant	None known	NA	+	Chr15:49,288,961–49,418,086	Obesity
Crabp1	54,882,658–54,891,163	Discordant	None known	NA	+	Chr15:76,419,749–76,427,622	No known phenotype
Man2c1	57,244,266–57,255,699	Discordant	None known	NA	+	Chr15:73,435,185–73,447,994	No known phenotype
Neil1	57,256,746–57,260,524	Discordant	None known	NA	+	Chr15:73,426,462–73,434,639	No known phenotype
Mpi1	57,658,200–57,666,660	Discordant	None known	NA	+	Chr15:72,969,914–72,977,130	Hepatic-intestinal
Bbs4	59,438,725–59,470,220	Discordant	None known	NA	+	Chr15:70,765,587–70,817,869	Obesity
Hexa	59,656,332–59,681,867	Discordant	None known	NA	+	Chr15:70,422,832–70,455,393	Tay-Sachs
Dpp8	65,154,659–65,204,852	Discordant	None known	NA	+	Chr15:63,526,027–63,597,095	No known phenotype
Anxa2	69,585,297–69,623,409	Discordant	None known	NA	+	Chr15:58,426,642–58,477,477	No known phenotype
Lipc	70,939,916–71,079,289	Discordant	None known	NA	+	Chr15:56,511,466–56,648,364	Obesity
Low priority
Pts	50,593,944–50,600,968	Discordant	rs29794257	G6D	−	Chr11:111,602,308–111,609,903	Hyperphenylalaninemia
Acat1	53,644,099–53,673,629	Concordant	None known	NA	+	Chr11:107,497,467–107,523,485	Ketoacidosis
AK006055	57,281,276–57,290,213	Discordant	rs13465819	G230S	−	Chr15:73,285,398–73,290,513	No known phenotype
Lsm16	57,822,503–57,864,085	Concordant	None knownb	NA	+	Chr15:72,709,952–72,775,413	No known phenotype
Gramd2	59,824,652–59,833,313	Discordant	rs29794257	R255C	−	Chr15:70,239,201–70,277,180	No known phenotype
Uaca	60,912,093–60,997,458	Discordant	rs3667578	Q425H	−	Chr15:68,733,947–68,842,904	No known phenotype
BC039571	67,790,284–67,791,775	Discordant	rs3721766	M127L	−	Chr15:60,243,408–60,244,436	No known phenotype
Vps13c	67,871,263–67,936,662	Discordant	rs3722443	T1502I	−	Chr15:59,931,879–60,139,939	No known phenotype

NA = not applicable.

Position in bp is given relative to Mouse Build 34. See text for details about haplotype blocks. Alleles are denoted by the amino acid substitution (using single letter codes) at the numbered position of the protein sequence, e.g., proline (P) is substituted for histidine (H) as position 312, P312H. Genes chosen for their functional relevance to obesity are denoted by a ‘‘+,’’ whereas those chosen only because they have a cSNP are denoted by ‘‘−.’’

^aThe allele was confirmed by direct sequencing of the parental strains (Accession Nos. DQ424862 and DQ517441).

^bThe protein-coding regions of this gene were sequenced to detect variants but none were found (Accession Nos. DQ240818 and DQ240819).

High priority is given to genes with a function related to obesity and known protein-coding variants. Medium priority is given to functional candidates within discordant haplotype blocks. Lower priority is given to functional candidates in concordant blocks or genes with protein coding variants but without an obvious functional relationship to obesity or gonadal depot weight. Within priority category, genes are ordered by location. Genes in transition areas between discordant and concordant blocks were categorized as discordant because the crossover points are not known. Human orthologs and their chromosome location in base pairs are relative to Build 35 of the human genome. When known, the human phenotype is listed in the right column (OMIM; see Website References).