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| Motility assay |
Mechanical measurement |
||||
|---|---|---|---|---|---|
| Velocity (μm/s) | Run length (μm) | Stall force (pN) | Unbinding force (pN)a |
||
| (−) | (+) | ||||
| WT | 0.49 | 0.84 | 5.6 | 7.8 | 5.7 |
| E410A | —b | —b | 3.5 | 5.3 | 5.2 |
| D417A | —b | —b | 3.0 | 3.8 | 6.2 |
| E421A | 0.45 | 0.66 | 3.1 | 3.6 | 5.6 |
| a Unbinding force obtained in AMP-PNP state in each minus- and plus-end loading direction. | |||||
| b Data not available because single kinesin molecules did not spontaneously bind to these microtubules. | |||||
