Figure 1.

Multiple alignment of the Tgs1 protein family. Conserved motifs are labeled according to the nomenclature described for the ‘classical’, Rossmann-fold AdoMet-dependent MTase superfamily (35). Invariant residues are highlighted in black, conserved residues are highlighted in gray. Residues studied by site-directed mutagenesis (in this work; 16) are indicated by ‘#’. Secondary structure (predicted for Tgs1, observed in Mj0882/1dus) is indicated. For Tgs1, regions predicted to be disordered in the absence of additional binding partners (for instance other proteins), are indicated by ‘x’.