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. 2003 Aug 15;31(16):4899–4909. doi: 10.1093/nar/gkg656

Figure 8.

Figure 8

Functional properties of Tgs1 point mutants. (A) Western analysis of GFP–Tgs1 point mutants. Equivalent amounts of cell extracts prepared from wild-type strains carrying the indicated GFP fusion constructs and grown at 28°C were fractionated by SDS–PAGE and immunoblotted with anti-GFP antibodies. Control extract was made from a wild-type strain carrying the GFP vector alone. The GFP–Tgs1 fusions are indicated by arrows. Additional bands correspond to anti-GFP antibodies cross-reacting polypeptides. (B) Growth assays. Exponentially growing cultures of yeast cells carrying the indicated GFP fusion mutant were serially diluted, spotted on plates and incubated at 16 and 28°C.