Table 1.
Genetic interaction of lin-66 with known heterochronic genes on alae formation
| Genotypea | Alae synthesis (%) (n) | ||
|---|---|---|---|
| L3 molt | L4 molt | Ad/L5 moltb | |
| WT (N2) | 0 (20) | 100 (20) | ND |
| lin-66 (ku423) | ND | 0 (14) | 100c (41) |
| lin-31 (n301); lin-66 (ku423) | ND | 0 (31) | 100c (15) |
| lin-31 (n301) | ND | 100% (31) | ND |
| lin-14 (ma135) | 100 (23) | ND | ND |
| lin-14 (ma135); lin-66 (ku423) | 0 (29) | 100c (25) | ND |
| lin-28 (n719) | 100 (18) | ND | ND |
| lin-28 (n719); lin-66 (ku423) | 100 (52) | ND | ND |
| hbl-1 (RNAi) | 83 (35) | 100c (15) | ND |
| lin-66 (ku423); hbl-1 (RNAi) | 0 (24) | 100c (35) | ND |
| lin-41 (ma104) | 60c (25) | 100c (25) | ND |
| lin-41 (ma104); lin-66 (ku423) | 33c (33) | 100c (36) | ND |
| lin-42 (n1089) | 80c (50) | 100c (38) | ND |
| lin-42 (n1089); lin-66 (ku423) | 3c (32) | 100c (21) | ND |
| lin-29 (n333) | ND | 0 (25) | 0 (28) |
| lin-29 (n333); lin-66 (ku423) | ND | 0 (28) | 0 (30) |
| lin-4 (e912) | ND | 0 | 0 |
| daf-12 (rh61) | ND | 100c (41) | ND |
| lin-66 (ku423); daf-12 (rh61) | ND | 0 (32) | 0 (42) |
| alg-1 (ok214) | ND | 59% (49) | 100% (16) |
| lin-66 (ku423); alg-1 (ok214) | ND | ND | 0% (33) |
| lin-46 (ma164) | ND | 100 | 100% |
| lin-66 (ku423); lin-46 (ma164) | ND | ND | 0% (26) |
| aThe newly synthesized cuticle was examined for the presence of alae by Nomarski microscope. | |||
| bAdult stage or fifth molting stage for retarded mutants that undergo an extra molting stage. | |||
| cSome animals had gaps in their alae. | |||
| ND, not determined. | |||