Table 2.
lin-66 (lf);daf-12 (rh61) and lin-66 (lf); alg-1 (lf) double mutants display a strong L2 reiteration phenotype
| No. of SCM∷GFP-positive cells/lateral side at each stage (n) | |||||
|---|---|---|---|---|---|
| L1 | L2 | L3 | L4 | Ad/L5 | |
| WT | 10 (7) | 16 (12) | 16 (11) | 16 (17) | 16 (19) |
| lin-66 (ku423) | 10 (22) | 16.5 (59) | 28.6 (39) | 29.1 (50) | 29.1 (25) |
| daf-12 (rh61) | 10 (10) | 16.6 (22) | 25.7 (21) | 26.6 (25) | 27.9 (14) |
| lin-66 (ku423); daf-12 (rh61) | 10 (12) | 17.0 (20) | 28.8 (25) | 50.6 (28) | 78 (6) |
| daf-12 (rh61rh411) | 10 (8) | 16.7 (8) | 19 (7) | 20.7 (14) | 21.5 (22) |
| lin-66 (ku423); daf-12 (rh61rh411) | 10 (6) | 16.2 (6) | 28.7 (6) | 49.4 (13) | 93 (7) |
| daf-12 (m20) | 10 (5) | 16.3 (8) | 18.3 (7) | 21 (7) | 19.6 (19) |
| lin-66 (ku423); daf-12 (m20) | 10 (8) | 16.5 (6) | 28.4 (7) | 48.7 (6) | 83.7 (10) |
| lin-4 (e912) | 10 (10) | 10 (12) | 10 (14) | 10 (6) | 10 (10) |
| lin-66 (ku423); alg-1 (ok214) | 9.8 (13) | 16.7 (12) | 27.7 (10) | 45.2 (6) | 83.2 (12) |
| lin-28 (RNAi) | ND | ND | ND | ND | 11.0 (25) |
| lin-66 (ku423); alg-1 (ok214); lin-28 (RNAi) | ND | ND | ND | ND | 11.7 (20) |
| lin-66 (ku423); daf-12 (rh61); lin-28 (RNAi) | ND | ND | ND | ND | 12.3 (13) |
| Seam cell nuclei were counted on one side of the animal of the indicated genotype and stage. | |||||
| RNAi was performed by injecting dsRNA into the adult gonad. The seam cell number of the next generation was counted. | |||||
| ND, not determined. | |||||