Figure 6.

Egr is involved in Mira telophase rescue. A schematic diagram showing the egr genomic locus (A). egr⁶⁶ deletes the entire coding region of egr. In egr⁶⁶ NBs, Mira (green) forms basal cortical crescents at metaphase (B) and segregates into the basal daughter cells at telophase (E). The asymmetric apical localization of DTRAF1 (red) seen in WT metaphase NBs (see Figure 1C) is disrupted and becomes cytoplasmic in egr⁶⁶ metaphase NBs (C). In egr insc NBs, Mira (green) is evenly distributed to the cell cortex of the majority of the metaphase NBs (D) and retained in the large cell at telophase (arrow, F and G). DNA is in blue. Apical is up. White dots outline the cell body.