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The HPLC analysis of the crude reaction mixture of the post-synthetic modification of precursors oligoribonucleotides into Ui6ACAUGUA (A) and Ums2i6ACAUGUA (B). Arrows indicate the positions of UACAUGUA (A) and Ums2ACAUGUA (B). Inserts indicate HPLC analysis of TLC-purified Ui6ACAUGUA (A) and Ums2i6ACAUGUA (B).
