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. Author manuscript; available in PMC: 2007 Nov 10.
Published in final edited form as: J Mol Biol. 2006 Aug 30;363(5):931–944. doi: 10.1016/j.jmb.2006.08.074

Fig. 3.

Fig. 3

σ/AsiA, σ/MotA, and AsiA/σ/MotA complexes in native protein gels. The indicated σ was incubated with excess His6AsiA and/or MotA and then subjected to electrophoresis through native 6 % acrylamide gels as described in Materials and Methods. The positions of σ, the σ/AsiA complex, the σ/MotA complex, and the AsiA/σ/MotA complex are shown. The positions of free MotA and AsiA are also marked. Excess free MotA protein is the dark band that migrates near the top of the gel in the lanes with added MotA. The excess free AsiA is usually not seen because AsiA is a small protein (10 kDa) and stains poorly.