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. Author manuscript; available in PMC: 2007 Nov 10.
Published in final edited form as: J Mol Biol. 2006 Aug 30;363(5):931–944. doi: 10.1016/j.jmb.2006.08.074

Fig. 5.

Fig. 5

The σ70 substitutions 551-552,554-555A (A) eliminate the interaction of the β-flap with σ70 region 4 whereas substitutions 584-588A (B) do not impair the interaction of σ70 region 4 with the β-flap. Cultures contained a pBRα-σ70 plasmid, which had σ70 region 4 (σ70 4) with the indicated substitutions, and either the control plasmid, pACλcI32, the pcI plasmid that lacks a fused bait protein, or the pcI-β-flap plasmid (β-flap). Graphs show β-galactosidase activity (in Miller units) versus the concentration of IPTG present in the cultures.