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. 2003 Aug 15;31(16):4805–4813. doi: 10.1093/nar/gkg661

Figure 4.

Figure 4

MBP–apoptin binding to DNA is sequence non-specific. MBP–apoptin was incubated with the λ/RsaI fragment pool in solution (10 mM NaCl). Protein–DNA complexes were recovered with amylose, and eluted with an NaCl step gradient. EB-stained 1.5% agarose gel, L = fragment pool, FT = flow-through, M = final wash, 2 M NaCl + 10 mM maltose. Any minor intensity differences in the overview gel (1.5% agarose) were not consistently reproducible upon adjusting the separation range of the gel to achieve higher resolution.