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Apoptin multimers contain about eight cooperative DNA-binding sites. (A) Titration of 12 bp dsDNA fragment (0.5 µg/ml, 65 nM fragment) with MBP–apoptin, |ΔF600| = reduction in ethidium fluorescence at 600 nm. (B) Titration of 177 bp blunt-ended dsDNA fragment (1 µg/ml, 8.7 nM fragment). Inset: emission spectra of 81 and 177 bp fragments (1 µg/ml) with and without 0.15 mg/ml MBP–apoptin.
