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. 2003 Aug 1;31(15):4597–4607. doi: 10.1093/nar/gkg638

Figure 2.

Figure 2

DNA binding of the heparin–agarose-purified yeast proteins assessed by EMSA. (A) The binding activity of heparin–agarose-purified yeast proteins for CLB2-L1 DNA (–933 to –514), which contains the UAS sequences, and CLB2-S DNA (–698 to – 662) containing the Mcm1p/Fkh2p/Ndd1p-binding sites, as well as STE2 and CLN3 UAS sequences, containing known Mcm1p-binding sites. The CLB2 DNAs are illustrated in Figure 1. Bound and free DNAs are indicated by arrows; the free CLB2-S DNA is not shown. (B) Binding of yeast heparin–agarose-purified proteins to 5 × 10–11 M labeled CLB2-L2 DNA (–933 to –622) was competed with unlabeled CLB2 DNA sequences from –825 to –622. Binding affinity was assessed by Scatchard analysis.