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. 2003 Aug 1;31(15):4597–4607. doi: 10.1093/nar/gkg638

Figure 6.

Figure 6

The CLB2 Reb1 site modulates Gal4p activation of gene expression. (A) Diagram of the four reporter constructs that were integrated at the URA3 locus and assayed in (B). The Gal4p DNA-binding site and the a-specific genes (asg) operator are present in the original construct (37). The asg operator was replaced by CLB2 sequences from –942 to –721 (either wild type or bearing the Reb1p site m1 mutation) or by a linker sequence as shown. (B) The expression of the CYC1-lacZ gene from yeast strains bearing each plasmid was examined on plates containing X-gal and galactose.