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. 2003 Aug 15;31(16):4929–4940. doi: 10.1093/nar/gkg663

Figure 8.

Figure 8

Expression of RUNX3 in cultured cells and human tissues. (A) Schematic figure of RUNX3 cDNA in which the relative positions of PCR primers and a 79 bp real-time RT–PCR product with primers (5′-ACTCAGCACCACAAGCCACT-3′ and 5′-GTCGGAGAATGGGTTCAGTTC-3′) are shown. (B) RT–PCR result of full-length RUNX3 mRNA expression in 143B and HeLa cells. A nested PCR strategy was performed to amplify the product at the N-terminal region of RUNX3 cDNA. Amplified product for the N-terminal region is only readily observed after second-round PCR, whereas amplified product for the C-terminal region is readily detectable. GAPDH is the internal control for sample loading. (C) RUNX3 mRNA is differentially expressed in 143B cells, HeLa cells and human tissues as revealed by real-time quantitative RT–PCR.