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. 2003 Aug 1;31(15):4317–4325. doi: 10.1093/nar/gkg640

Figure 2.

Figure 2

CSP41 binds to petD RNA in the absence of divalent metals. Inset: gel mobility shift assay of petDΔ3 binding by CSP41. Uniformly labeled petDΔ3 RNA at the indicated concentrations was incubated with 5 µg of CSP41. The samples were then treated with 40 U of RNase T1 and analyzed in a 5% native polyacrylamide gel. Reactions were performed in duplicate. The intensity of the shifted band from the gel mobility shift assay was plotted versus the concentration of petDΔ3 RNA. The error in intensity at each data point was typically <5%. The curve represents the best fit to equation 2 (see Materials and Methods).