Table 2. Effects of ErmC′ mutations on erythromycin resistance.
ErmC′ variant | Erythromycin MIC (mg/l) |
---|---|
Negative control (empty pUC18) | 80 |
wt | >2560 |
T108A | 640–1280 |
R112A | 1280 |
R112D | 640–1280 |
K133A | 1280 |
R134A | 80 |
R140A | 640–1280 |
K197A | >2560 |
N192A | 2560 |
M196A | >2560 |
N200A | >2560 |
E202A | 2560 |
K204A | 2560 |
K205A | 2560 |
K209A | >2560 |
Clustera | 2560 |
Δ182–244 | 80 |
Δ193–203 | 80 |
Δ207–219 | 80 |
Δ, indicated deletion.
aCluster, K197A/N200A/E202A/K204A/K205A.