Table 3. Summary of the in vitro characterization of ErmC′ variants.

Mutants	Kdapp (M × 10–7)	AdoMet			RNA
		KM (M × 10–6)	kcat (s–1 × 10–2)	Relative kcat/KM	KM (M × 10–6)	kcat (s–1 × 10–3)	Relative kcat/KM
wt	1.24 ± 0.29	2.82 ± 0.20	6.55 ± 0.10	1.00	0.69 ± 0.17	6.37 ± 0.42	1.00
T108A	6.41 ± 1.67	25.67 ± 5.24	2.29 ± 0.22	0.004	0.43 ± 0.07	0.11 ± 0.004	0.03
R112A	2.03 ± 0.61	5.80 ± 1.13	1.61 ± 0.09	0.12	1.70 ± 0.39	1.13 ± 0.09	0.07
R112D	4.22 ± 0.99	10.76 ± 2.92	1.21 ± 0.12	0.005	3.64 ± 1.92	0.49 ± 0.11	0.02
K133A	1.29 ± 0.47	6.00 ± 0.68	3.80 ± 0.13	0.27	1.86 ± 0.72	2.22 ± 0.33	0.13
R134A	2.46 ± 0.67	21.32 ± 7.57	1.57 ± 0.25	0.0032	2.50 ± 1.37	0.21 ± 0.05	0.01
R140A	2.98 ± 0.98	22.28 ± 10.28	1.49 ± 0.31	0.014	1.41 ± 0.35	0.34 ± 0.06	0.03
N192A	1.72 ± 0.42	2.00 ± 0.18	3.41 ± 0.06	0.73	0.79 ± 0.09	5.44 ± 0.17	0.74
M196A	0.76 ± 0.21	3.28 ± 0.38	3.18 ± 0.06	0.42	0.62 ± 0.05	3.18 ± 0.12	0.56
K197A	1.23 ± 0.22	3.34 ± 0.64	5.22 ± 0.23	0.67	1.59 ± 0.26	7.31 ± 0.42	0.50
N200A	1.18 ± 0.33	7.09 ± 0.52	6.88 ± 0.16	0.42	0.89 ± 0.18	4.39 ± 0.26	0.54
E202A	0.88 ± 0.23	9.77 ± 2.33	7.35 ± 0.62	0.32	0.57 ± 0.06	2.11 ± 0.05	0.40
K204A	1.27 ± 0.32	3.83 ± 0.89	6.48 ± 0.37	0.73	0.89 ± 0.23	7.29 ± 0.54	0.89
K205A	1.25 ± 0.29	4.43 ± 0.86	6.49 ± 0.33	0.63	0.91 ± 0.23	7.66 ± 0.57	0.92
K209A	1.24 ± 0.34	4.62 ± 0.72	7.75 ± 0.32	0.72	0.95 ± 0.23	6.37 ± 0.47	0.73
Clustera	1.48 ± 0.46	6.56 ± 1.60	3.14 ± 0.23	0.21	1.21 ± 0.28	1.93 ± 0.14	0.17

^aCluster, K197A/N200A/E202A/K204A/K205A.