Figure 3.

The str1⁺ promoter GATA element confers iron responsiveness to the minimal promoter CYC1-lacZ. (A) CYC1-lacZ fusion genes harboring a wild-type (WT) DNA fragment and a GATA mutant (Mutant) derived from the str1⁺ promoter were assayed. The lacZ and act1⁺ mRNA levels are indicated by arrows. Results shown are representative of three independent experiments. (B) The gray box represents the wild-type 5′-AGATAA-3′ element and the filled box indicates the mutant element containing multiple point substitutions (5′-GCCGTC-3′ instead of 5′-AGATAA-3′). The nucleotide numbers refer to the position relative to the A of the ATG codon of the str1⁺ ORF.