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. 2003 Aug 15;31(16):4864–4873. doi: 10.1093/nar/gkg674

Figure 3.

Figure 3

Repeated DNA length polymorphism within and between cloned stocks of T.brucei. Columns 1, 4, 7, 10 = Tb927; columns 2, 5, 8, 11 = Tb247; columns 3, 6, 9, 12 = Tb427. L = 100 bp ladder. (A) Restriction fragments containing tandemly arrayed DNA sequences. Top left, tubulin gene array (unit size 3641 bp): lanes 1–3, HpaI/NcoI; lanes 4–6, SspI/XmnI, fragment lengths in Tb927chrIa sequence file 16 235 and 16 247 bp, respectively (but not represented in full, see Fig. 1), length polymorphism between stocks ∼35 kb. Top right, histone H3 gene array (unit size 1584 bp): lanes 1–3, Sau3AI; lanes 4–6, PstI/EcoRI, fragment lengths in sequence file 11 469 and 11 664 bp, respectively, length polymorphism ∼30 kb. Bottom left, Family 15 tandem array (unit size 3100 bp): lanes 1–3, SspI; lanes 4–6, BglII, fragment lengths in sequence file 18 171 and 26 134 bp, length polymorphism ∼15 kb. Bottom right, 123-bp repeat array in Tb927.1.1740: lanes 1–3, HindIII/SspI; lanes 4–6, XmnI, fragment lengths in sequence file 20 838 and 23 880 bp, length polymorphism ∼15 kb. (B) Length polymorphism of PCR amplification products containing microsatellite. Left to right: msat D2 in Tb.1.1780 and msats E5, 18 and 15 in intergenic sequence.