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Secondary structure of S.cerevisiae tRNAArg4CCG. Numbering is according to Sprinzl et al. (32). The encircled nucleotides are the mutations found at corresponding positions. Red filled circles indicate the lethal mutations that gave inactive tRNAs in vivo. They exhibited a non-sectored red phenotype. Empty circles contained nucleotides that gave active tRNAs in vivo (red-white sectored phenotype).
