Figure 3.

Stability of the BAC DNA after recombineering. An agarose gel picture of various BAC DNA samples digested with EcoRV and SpeI restriction enzymes showed the absence of any unwanted rearrangement after the genetic manipulation. Lane 1 contains the parental BAC HB1-812 DNA, lanes 2 and 3 contain ‘intermediate BAC’ DNA from two different clones and lanes 4–6 contain DNA from three different mutant (Delt A) BAC clones. A 1 kb plus DNA ladder (Invitrogen) was used as molecular weight marker (M). The smallest marker band on the gel represents an 850 bp fragment.