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Direct interaction of Rpb7 with Seb1. Rpb7 was mixed with GST or GST–Seb1 and incubated with glutathione–agarose beads. One-tenth of input (I), final wash fraction of the same volume with elution (W) and elution fraction with glutathione (E) were separated by 12.5% SDS–PAGE, transferred to a PVDF membrane, and probed with anti-Rpb7 antibody. Immunoblotting with anti-GST antibody confirmed the presence of GST and GST–Seb1 (data not shown).
