Figure 3.

Representative polony images. Lys131Glu mutant time series from left to right: (A) 0 h, (B) 51.75 h and (C) 97 h. Polonies were initially extended with cyanine-5-dCTP (A and C) or cyanine-5-dUTP (B). The slides were then imaged and the extended primers were removed. The procedure was then repeated with cyanine-5-dCTP used to extend (B) and cyanine-5-dUTP used to extend (A) and (C). A positive extension with cyanine-5-dUTP indicates that the wild-type nucleotide is present at position 391 of PGK1 (red), while positive extension with cyanine-5-dCTP indicates the mutant nucleotide (green) at this position.