Table 2. Comparison of specific growth rates of PGK1 mutants measured in individual shake flask experiments and calculated from the initial competitive growth experiment.
Mutant (CYC1 promoter) | µmeasured (per h) | µcalculated (per h) |
---|---|---|
Wild-type PGK1 | 0.266 ± 0.024 | 0.266a |
Arg65Ser PGK1 | 0.252 ± 0.010 | 0.250 |
Lys131Glu PGK1 | 0.264 ± 0.004 | 0.261 |
Glu190Gln PGK1 | 0.177 ± 0.001 | 0.220 |
His388Glu PGK1 | 0.249 ± 0.020 | 0.251 |
aSupplied to the curve fitting routine as a fixed value.