Skip to main content
. 2003 Aug 1;31(15):e85. doi: 10.1093/nar/gng085

Figure 1.

Figure 1

Plasmid pIR-EGFP and Southern blot analysis of transgenic lines. (a) Schematic representation of transformation vector pIR-EGFP. Actin5C, D.melanogaster Actin5C promoter; HspT, D.melanogaster Hsp70 terminator sequence; ML, minos left arm; MR, minos right arm; IR-EGFP, EGFP inverted repeat; DsRed, DsRed selectable marker. The restriction sites (H, HincII; E, EcoRI; N, NotI) and the probes (M, EGFP-S and HST, represented by black bars) used in the Southern blot analyses are indicated. A 1 kb scale marker is shown. (b) Southern blot analyses of genomic DNA from 17 transgenic lines digested with HincII and hybridized with probe M. (c) Southern blot analyses of genomic DNA from selected lines eIR-A1, eIR-A2, eIR-A3, eIR-B1, eIR-C2 and eIR-191, digested with EcoRI/NotI and hybridized with probe EGFP-S (upper) or probe HST (lower). The size marker is in kilobases (kb). A2, eIR-A2; A3, eIR-A3; B1, eIR-B1; 191, e-IR-191; A1, eIR-A1; C2, eIR-C2.