Figure 2.

Images of hybridisation of a DNA probe spotted on activated glass slides with dendrimer generation 4 in the absence or in the presence of DMSO. (A) PCR products from two yeast ORFs (CDC19/YAL038w and CLN3/YAL040c) were resuspended in Na₂ phosphate buffer 0.3 M, pH 9 in the absence or in the presence of DMS0 50%. (B) A 5′-NH₂ 35mer oligonucleotide was prepared in the same buffer at two different concentrations (5 and 10 µM) in the absence or presence of increasing concentration of DMSO. DNAs were spotted in triplicate on the activated dendrislides and hybridisation was carried out with yeast labelled cDNA (A) or with a complementary Cy5-labelled 15mer (5′-Cy5-AATGGTATCGGAGTA-3′) (B).