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. 2003 Aug 15;31(16):e88. doi: 10.1093/nar/gng088

Figure 5.

Figure 5

Detection sensitivity of dendrislide compared with commercially available activated glass slides. A single-stranded 35mer amino-oligonucleotide (5′-NH2-GTGATCGTTGTATCGAGGAATACTCCGATACCATT-3′) at a concentration of 10 µM was spotted on the different functionalised glass slides. The target Cy5-labelled 15mer oligonucleotides (5′-Cy5-AATGGTATCGGAGTA-3′) were hybridised at concentrations ranging from 0.001 to 100 nM. Image capture was measured at 635 nm with GenePix 4000A at 500 PMT. The value of fluorescence intensity reported is the fluorescent signal – background.