Table 1. Absolute quantification of selected genes in HL-60 cDNA.
| Accession no. | Gene | Gene concentrationa | Gene copies/µg total cDNAb |
|---|---|---|---|
| BC009275 | β-Actin | 1330 ± 260 pM | (8.0 ± 1.6) × 109 |
| L41490 | EL-1 | 810 ± 170 pM | (4.9 ± 1.0) × 109 |
| M81757 | S19 | 660 ± 70 pM | (4.0 ± 0.4) × 109 |
| K00558 | tuba | 115 ± 30 pM | (7.0 ± 1.7) × 108 |
| M28209 | RAB1 | 50 ± 13 pM | (3.0 ± 0.8) × 108 |
| X95325 | DNAbp | 38 ± 3.3 pM | (2.3 ± 0.2) × 108 |
| V00572 | PGK1 | 12.8 ± 3.3 pM | (0.8 ± 0.2) × 108 |
| L19067 | p65 | 2.1 ± 1.3 pM | (1.3 ± 0.8) × 107 |
aMean ± SD in 1 µl assay volume using 100 and 200 ng of HL-60 cDNA, respectively. The concentration obtained for 200 ng was divided by 2.
bMean ± SD.