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. 1976 Jul;32(1):159–165. doi: 10.1128/aem.32.1.159-165.1976

Detection of Thiobacillus ferrooxidans in acid mine environments by indirect fluorescent antibody staining.

W A Apel, P R Dugan, J A Filppi, M S Rheins
PMCID: PMC170021  PMID: 61736

Abstract

An indirect fluorescent antibody (FA) staining technique was developed for the rapid detection of Thiobacillus ferrooxidans. The specificity of the FA stain for T. ferrooxidans was demonstrated with both laboratory and environmental samples. Coal refuse examined by scanning electron microscopy exhibited a rough, porous surface, which was characteristically covered by water-soluble crystals. Significant numbers of T. ferrooxidans were detected in the refuse pores. A positive correlation between numbers of T. ferrooxidans and acid production in coal refuse in the laboratory was demonstrated with the FA technique.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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