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. 2006 Dec 14;103(52):19635–19640. doi: 10.1073/pnas.0609513103

Fig. 2.

Fig. 2.

The polymerase extension scheme (Left) and MALDI-TOF MS spectra of the four consecutive extension products and their deallylated products (Right). Primer extended with 3′-O-allyl-dUTP-allyl-R6G (1), and its deallylated product 2; Product 2 extended with 3′-O-allyl-dGTP-allyl-bodipy-650 (3), and its deallylated product 4; Product 4 extended with 3′-O-allyl-dATP-allyl-ROX (5), and its deallylated product 6; Product 6 extended with 3′-O-allyl-dCTP-allyl-bodipy-FL-510 (7), and its deallylated product (8). After 30 s of incubation with the palladium/TPPTS mixture at 70°C, deallylation is complete with both the fluorophores and the 3′-O-allyl groups cleaved from the extended DNA products.