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CD spectra of acetoin produced by the E636A and E636Q PDHc-E1 variants and the E477Q and D28A YPDC variants. (a) PDHc-E1 E636Q, (b) PDHc-E1 E636A, (c) YPDC D28A, (d) YPDC E477Q. (λmax= 278 nm). Conditions are described under Materials and methods.
