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CD spectra of 1,3,4-trihydroxy-2-butanone produced by parental PDHc-E1 and its E636A and E636Q variants and the E477Q YPDC variant. (a) PDHc-E1 E636Q + β-hydroxypyruvate and glycolaldehyde, (b) PDHc-E1 E636A+β-hydroxypyruvate and glycolaldehyde, (c) PDHc-E1 + β -hydroxypyruvate, (d) YPDC E477Q + β-hydroxypyruvate and glycolaldehyde. (λmax= 278 nm). Conditions are described under Materials and methods.
